Pb7wgf2
Splet1.构建10kb大小，具有绿色荧光蛋白的质粒载体pb7wgf2.0； 2.用qiagen公司生产的qiafilter tm plasmidmidikit试剂盒制备质粒dna；设置质粒dna浓度为600ng/μl； 3.用sigma-aldrich … Splet05. jul. 2012 · pB7WGF2/GFP-AtGRXS17 was introduced into Agrobacterium strain GV3101 as described and then used for agro-infiltration as previously described (Liu et al., 2005). For heat shock treatment, at 1.5–2 days postinfiltration (DPI), the infiltrated leaves were detached from tobacco plants, kept in Petri dishes with the moistened filter paper and ...
Pb7wgf2
Did you know?
Splet03. mar. 2024 · For colocalization, pB7WGF2 plasmids containing EGFP-CRN C-terminal construct fusions and pGWB461 plasmids containing tagRFP-SlTCP14 fusions were transformed into Agrobacterium tumefaciens strain AGL1. For BiFC, CRN and TCP constructs were transformed into pCL112 and pCL113. Transformants were grown on … Splet03. avg. 2024 · The pB7WGF2-BPC6 construct was transformed into Agrobacterium tumefaciens strain GV3105 via electroporation. The A. tumefaciens cells that contained pB7WGF2-BPC6 were then introduced into WT. The resulting T1 pB7WGF2-BPC6 transgenic plants were selected by BASTA as described previously (Sun et al., 2016). Homozygous …
Splet19. dec. 2024 · The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. … Splet24. maj 2024 · The sequences were mobilized into pBGWFS7, pB7FWG2 or pB7WGF2 (ref. 31) to generate promoter:GFP–GUS reporter lines and p35S promoter driven C- and N-terminal translational GFP fusion constructs ...
Splet07. jul. 2024 · For transient expression of GFP-fusion proteins, the genomic sequences of GRP7, PTB3, RH11, a putative TNTase and a putative endonuclease were amplified from genomic DNA and cloned into pB7WGF2. DCP5 was amplified from genomic DNA, RH6, RH8 and RH12 coding sequences were amplified from cDNA and all cloned in pUB10-GFP. Splet19. dec. 2024 · A conserved residue in Stphot1, Asp at position 832 (D832), was mutated to Asn (N) generating Stphot1.KD, which results in a kinase-dead version of Stphot1. The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. Agrobacterium …
Splet03. apr. 2024 · The full AtMiro2 coding sequence was cloned using Gateway reactions (Invitrogen) into the binary destination vector PB7WGF2 creating an N-terminal GFP fusion. Using this as template, the ...
Splet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip method for plant transformation (Clough and Bent, 1998). Overexpresser lines were selected by spraying a 0.0114% glufosinate ammonium (BASTA, Bayer®, Whippany, NJ, … the cold forging processSplet26. jul. 2024 · The mutant StNRL1 was recombined, using LR clonase, into pB7WGF2, PGWB412, or PGWB18 for in planta assays. The Pi02860 in which we removed the 10 or 5 amino acids in the C terminus were cloned using PUC57-Pi02860 without Signal peptide as a template, generating pDonr201-Pi02860 ∆126–135 or pDonr201-Pi02860 ∆131–135 . the cold food festivalSplet23. nov. 2024 · Agrobacterium tumefaciens GV3101 transformed with pB7WGF2.0 or pK7WGR2.0 harboring each gene of interest or empty (as control) were cultivated … the cold full moonhttp://www.biovector.net/product/1684236.html the cold gameSplet23. okt. 2024 · For SlACRE75, SlACRE180, NbACRE75 and NbACRE180, cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and … the cold general’s fierce wifeSplet21. nov. 2024 · The GFP-OsGRXS17 construct was produced by an LR clonase reaction between the entry vector and pB7WGF2 51. As a control, a modified green fluorescent … the cold geniusthe cold god