Optiphase supermix
WebOptiphase ‘SuperMix’ scintillation fluid was from Perkin Elmer Life Sciences. Bacterial Artificial Chromosome Recombineering and Transgenesis A bacterial artificial chromosome (BAC) RP24-149I12 containing the mouse α6 nicotinic receptor subunit gene (Chrna6) was obtained from the BACPAC Resource WebNov 18, 2024 · 加入闪烁 液后置于液闪计数仪(OptiPhase SuperMix,PerkinElmer)读取同位素的信号，以CPM (Counts Per Minute)表示。 下游cAMP信号检测 稳定表达的FlpInCHO细胞接种于6孔细胞培养板过夜培养，以每孔8000个细 胞的浓度转接入384孔板中培养24小时。
Optiphase supermix
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Web0.15 ml Optiphase “SuperMix” scintillation mixture. Instrument efficiency was 40%. Perfusion data were plotted as counts per minute versus fraction number. Fractions col-lected before and after the peak were used to calculate baseline as a single exponential de-cay. The calculated baseline was subtracted from the experimental data ... WebToggle navigation. Products & Services Analysis & Characterization >. Analysis & Characterization
WebJan 29, 1999 · The bound IP was eluted with 1.0 M ammonium formate in 0.1 M formic acid. Fifty microliters of the eluate from each well was transferred into a Wallac 96-well plate … WebAug 20, 2016 · The medium (100 μl) was then transferred to PET 24 well sample counting plates containing 300 μl OptiPhase SuperMix scintillation cocktail for counting. Fresh medium (0.5 ml) was applied to the cells, and then removed after 0 , 0.5, 1, 2, 5, 15, 60 and 1440 min of exposure. 100 μl aliquots of the fresh medium were transferred to ...
WebOct 22, 2024 · [3H]-Estradiol 17β-D-glucuronide and Optiphase Supermix liquid scintillation cocktail and 24-well liquid scintillation counting visiplates were purchased from PerkinElmer Life and Analytical Sciences (Buckinghamshire, UK). Hanks balanced salt solution (Gibco HBSS; containing CaCl 2 and MgCl 2 ), Dulbecco’s modified eagle medium ... WebSep 1, 2016 · RNA binding YSi SPA beads and OptiPhase SuperMix scintillation cocktail were purchased from PerkinElmer. UniverSol-ES liquid scintillation cocktail was purchased from MP Biomedicals (Santa Ana, CA). Bovine serum albumin (BSA) fraction V, fatty acid-free, was purchased from Roche (Basel, Switzerland).
WebJan 31, 2024 · OptiPhase SuperMix ® liquid scintillation cocktail (purchased from PerkinElmer Life Sciences, Waltham, MA). 10. 30% glacial acetic acid. 11. Pierce Bradford …
WebDec 25, 2016 · They include assays to detect the possible temporal separation between membrane permeabilization and bacterial killing events, to assess the capacity of antimicrobial peptides to cross the bacterial membranes and reside in the cytoplasm, and later to inhibit vital cell functions such as DNA transcription and protein translation. Key … how to spell natural disasterWebMay 27, 2016 · The Optiphase™ supermix and Hionic-Fluor™ liquid scintillation cocktails and Solvable™ were purchased from Perkin Elmer (Coventry, UK). Liposomal amphotericin B (AmBisome®, Gilead Sciences, Foster City, CA, USA) and amphotericin B deoxycholate (Fungizone®, ER Squibb, Uxbridge, UK) were reconstituted according to manufacturer's … rds aliyun_rootWebJul 21, 2010 · Optiphase ‘SuperMix’ scintillation fluid was from PerkinElmer Life Sciences. Results. α6α4* nAChR-mediated locomotion and behavioral analysis. α6 L9′S BAC transgenic mice use the full α6 promoter to drive the expression of a leucine 9′ to serine mutation in the ion channel pore lining transmembrane ... rds ahlWebOptiphase Supermix scintillation cocktail (2.5mL) was added to each sample. Triplicate samples of 100mL aliquots of the perfusion medium were treated in the same manner as the perfused brain samples. All samples were then measured for disintegrations per minute (dpm) (1450 LSC and Luminescence Counter; Perkin Elmer, Waltham, MA, USA). how to spell naughtseysWebSep 18, 2007 · After the effector cell addition, the cells were incubated 12–16 h before being harvested, when 25 μl of cell supernatant was added to 175 μl of OptiPhase SuperMix scintillation fluid (Wallac, Loughborough, Leicestershire, U.K.) and analyzed with a MicroBeta reader (PerkinElmer, Wellesley, MA). how to spell naturalistWebylamide (LSD), [3H]-melatonin, and OptiPhase SuperMix in the assay were purchased from Perkin-Elmer Life Sciences (NEN). Human MT 1 /MT 2 receptor membrane proteins were also Perkin-Elmer FIGURE 1 Chemical structure of agomelatine (A), structure of the series of derivatives of agomelatine (B), and GW117 (C) rds alicloudWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health … how to spell naught