Hct116 puromycin
WebSep 16, 2024 · To examine the role of CTRP1 in tumor progression, we generated CTRP1 knockout A549 and HCT116 cell lines, which reduced the expression levels of nuclear factor (NF)-κB-dependent and metastasis-promoting transcripts. ... and cells were selected with puromycin (2 µg/mL). Control cells were generated by transduction of an empty vector in ... WebJan 10, 2024 · Following puromycin selection we identified single clones of DLD-1 and HCT116 cells displaying a green fluorescence, which were isolated and expanded as monoclonal cell lines (Figure 6A). To confirm correct CB integration at the AAVS1 locus we performed PCR-based genotyping as illustrated in Figure 6 B (strategy outlined in Figure …
Hct116 puromycin
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WebHCT116-hNIS-Neo/Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells were transduced with LV-hNIS-Neo (LV013) and LV-Fluc-P2A-Puro (LV012) and selected using G418 and puromycin. WebNational Center for Biotechnology Information
WebJan 1, 2016 · Homeowners aggrieved by their homeowners associations (HOAs) often quickly notice when the Board of Directors of the HOA fails to follow its own rules, or … WebApr 24, 2024 · Notably, puromycin upregulated the expression of RPL5 and RPL11 to directly bind to MDM2 in HCT116 cells. Conversely, deletion of RPL5 and RPL11 …
http://cell-lines.toku-e.com/Cell-Lines_1422.html WebAug 29, 2002 · A colorectal carcinoma cell line, HCT116, which has both mutated and wild-type β-catenin genes, was engineered by homologous recombination to investigate the significance of β-catenin gene mutation.
WebPuromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. During puromycin selection, this antibiotic inhibits cellular protein synthesis by …
WebMar 14, 2024 · Puromycin was bought from Sigma-Aldrich (St. Louis, MO, USA). Firefly luciferase-lentiviruses and Polybrene were obtained from GENECHEM (Shanghai, China). D-luciferin potassium salt was obtained from PROMEGA (Madison, WI, USA). ... Compared to HCT116 and SW620 tumor-bearing mice, other groups (SW480, HT29, LOVO and … merrick bank cd rates today jumboWebPuromycin titration in HCT116 cells. Cells were treated with given concentrations of puromycin. Cell death was microscopically monitored over time. Scale bar: 50 µm. 4 x … merrick bank ceo addressWebHCT116-Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells … merrick bank ceo email addressWebHCT 116 Cell Line. HCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into … merrick bank ceoWebDec 30, 2024 · Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose product, puromycin acetyltransferase, inactivates puromycin via acetylation; recommended concentration in cell culture 1-10µg/ml. Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of … how rich is kenny logginsWebOct 28, 2013 · HCT116 TCF4/Wnt cells were cultured in the presence of 50 μg ml −1 hygromycin B, and HCT116 TCF/LEF cells were cultured in the presence of 10 μg ml −1 puromycin. Primary colon cancer ... how rich is kelsey grammerWebMar 25, 2024 · Next, we constructed knockout plasmid by cloning the sgRNA into the pCasCMV-Puro-U6 vector. After transfection of the plasmid into HCT-116 cells, TrxR1 knockout HCT-116 cells were selected using puromycin resistance. The TrxR1 knockout efficiency was identified and verified by DNA sequencing, immunoblotting, TRFS-green … merrick bank charge card login