Determination of aconitase activity
WebApr 14, 2024 · Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities. The chemical composition of the hydroethanolic extracts, obtained from dried plants of S. incarnata, S. coccinea, and … WebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent …
Determination of aconitase activity
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WebJan 10, 2024 · This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. The protocol is optimized for … WebEscherichia coli possesses two aconitases, a stationary-phase enzyme (AcnA), which is induced by iron and oxidative stress, and a major but less stable enzyme (AcnB), synthesized during exponential growth. In addition to the catalytic activities of the holo-proteins, the apo-proteins function as post-transcriptional regulators by site-specific …
WebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice …
WebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … WebApr 10, 2024 · This piece explains an activity that allows for the simple and accurate determination of the heat of vaporization, ΔH vap, of water at 100°C, and ultimately the approximate strength of a hydrogen bond in boiling water, in kJ·mol –1. The vaporization of water is an endothermic process represented by Equation 1:
WebMitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative... Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Springer for Research & Development
WebSep 17, 2024 · These intermediates are capable of oxidizing the cluster, which leads to iron release and consequent loss of the catalytic activity of aconitase. As the reaction of the Fe-S cluster with O 2 •-is fast (∼10 7 M-1 s-1), quite specific, and reversible in vivo, quantification of active aconitase has been used to evaluate O 2 •-formation in cells. pho msp discordWebAug 31, 1999 · The aconitase protein of Bacillus subtilis was able to bind specifically to sequences resembling the iron response elements (IREs) found in eukaryotic mRNAs. The sequences bound include the rabbit ferritin IRE and IRE-like sequences in the B. subtilis operons that encode the major cytochrome oxidase and an iron uptake system. IRE … pho miss saigon hershey paWebDec 10, 2024 · Mitochondrial Aconitase In-Gel Enzyme Activity Assays Protein samples were separated using a non-denaturing gel, and then the gel was incubated in a coloring … pho mud serverWebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added … how do you calculate bond energiesWebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by … pho msp youtubeWebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been … how do you calculate btuWebJul 1, 2007 · We examined the hydroxyketone-mediated production of reactive oxygen species by determination of the aconitase activity. Aconitase is the most sensitive enzyme to reactive oxygen species, and thus, we can estimate the generation of superoxide radical by the inactivation of the enzyme (Gardner and Fridovich, 1992, Murakami and … how do you calculate capacity in scrum